primary antibodies against cd206, na+-k+ atpase, arg-1  (Cell Signaling Technology Inc)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: An engineered adipose formulation decreases hepatic inflammation and fibrosis in a rodent model of metabolic dysfunction-associated steatotic liver disease

doi: 10.3389/fbioe.2025.1579062

Figure Lengend Snippet: Effects of mAF treatment on hepatic macrophages in CGI-58 LivKO mice. (A) Representative IHC images and quantification of the (B) M1 macrophage marker CD68, (C) M2 macrophage marker CD206, and (D) ratio of CD68 to CD206 in the liver of mice. Data are presented as mean ± SD. n = 8 for treatment group, and n = 3 for ALB- group. *P < 0.05, **P < 0.01 by Student’s t-test. Scale bars: 50 μm.

Article Snippet: The expression of CD68 and CD206 in the liver was evaluated by immunohistochemical staining using primary antibodies against mouse CD68 (1:4,000 dilution, Abcam, Waltham, MA) or mouse CD206 (1:1,600 dilution, Cell Signaling Technology ® , Danvers, MA).

Techniques: Marker

Journal: Bioactive Materials

Article Title: An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration

doi: 10.1016/j.bioactmat.2025.03.027

Figure Lengend Snippet: Construction of Engineered Exos. (A) Representative confocal microscopic images of BMSC Apts after co-culture with BMSCs for 4 h. Red: AF647-labeled Apt; green: F-actin; blue: DAPI-labeled cell nuclei. (B) Flow cytometry analysis was performed to detect the specificity of AF647-labeled BMSC Apts. (C) Flow cytometry analysis of M2 macrophage markers F4/80 and CD206. (D) Confocal microscopic image of DiO-labeled Exos cocultured with BMSCs. (E) WB analysis of exosomal positive and negative markers in Exos and donor cells. (F) Nanoflow cytometry analysis of the binding of Apt nanoparticles to Exos. (G) WB analysis of exosomal positive and negative markers in Exo and Apt-NP-Exo groups. (H) Nanoflow cytometry analysis of size distribution of Exos and Apt-NP-Exos. (I) TEM images of Exos and Apt-NP-Exos.

Article Snippet: Tissue sections were incubated overnight at 4 °C with primary antibodies against CD68, CD80, and CD206 (ServiceBio, China).

Techniques: Co-Culture Assay, Labeling, Flow Cytometry, Cytometry, Binding Assay

Journal: Bioactive Materials

Article Title: An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration

doi: 10.1016/j.bioactmat.2025.03.027

Figure Lengend Snippet: PEC-Apt-NP-Exo Regulating the Early Immune Environment of Bone Defects. Immunofluorescence staining of (A) M2 macrophages (red: CD68; green: CD206; blue: nuclei) and (B) M1 macrophages (red: CD68; green: CD80; blue: nuclei) one week after the implantation of various membranes into critical-sized calvarial bone defects in mice. (C) Quantification of the number and ratio of M2 macrophages to M1 macrophages. Data were shown as mean ± SDs. ns, no significance, ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05.

Article Snippet: Tissue sections were incubated overnight at 4 °C with primary antibodies against CD68, CD80, and CD206 (ServiceBio, China).

Techniques: Immunofluorescence, Staining